The Laboratory of Tom Walz

Publications

2008

Gonen, T., Hite, R. K., Cheng, Y., Petre, B. M., Kistler, J. & Walz, T. Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20. J. Mol. Biol. 376: 380-392 (2008).

Li, Z., Strunk, J. J., Lamken, P., Piehler, J. & Walz T. The EM structure of a type I interferon-receptor complex reveals a novel mechanism for cytokine signaling. J. Mol. Biol. 377: 715-724 (2008).

Strunk, J. J., Gregor, I., Becker, Y., Li, Z., Gavutis, M., Jaks, E., Lamken, P., Walz, T., Enderlein, J. & Piehler, J. Ligand binding induces a conformational change in ifnar1 that is propagated to its membrane-proximal domain. J. Mol. Biol. 377: 725-739 (2008).

Kelly, D. F., Dukovski, D. & Walz, T. Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy. Proc. Natl. Acad. Sci. U. S. A. 105: 4703-4708 (2008).

Engel, A., Fujiyoshi, Y., Gonen, T. & Walz, T. Junction-forming aquaporins. Curr. Opin. Struct. Biol. 18: 229-235 (2008).

Stahlberg, H. & Walz, T. Molecular electron microscopy: state of the art and current challenges. ACS Chem. Biol. 3: 268-281 (2008).

Hite, R. K., Gonen, T., Harrison, S. C. & Walz, T. Interactions of lipids with aquaporin-0 and other membrane proteins. Pflugers Arch. 456: 651-661 (2008).

Hartley, D. M., Zhao, C., Speier, A. C., Woodard, G. A., Li, S., Li, Z. & Walz, T. Transglutaminase induces protofibril-like amyloid beta-protein assemblies that are protease-resistant and inhibit long-term potentiation. J. Biol. Chem. 283: 16790-16800 (2008).

Wu, W., Sinha, D., Shikov, S., Yip, C. K., Walz, T., Billings, P. C., Lear, J. D. & Walsh, P. N. Factor XI homodimer structure is essential for normal proteolytic activation by factor XIIa, thrombin, and factor XIa. J. Biol. Chem. 283: 18655-18664 (2008).

Skiniotis, G., Lupardus, P. J., Martick, M., Walz, T. & Garcia, K. C. Structural organization of a full-length gp130/LIF-R cytokine receptor transmembrane complex. Mol. Cell 31: 737-748 (2008).

Mi, L. Z., Grey, M. J., Nishida, N., Walz, T., Lu, C. & Springer, T. A. Functional and structural stability of the epidermal growth factor receptor in detergent micelles and phospholipid nanodiscs. Biochemistry 47: 10314-10323 (2008).

Kelly, D. F., Abeyrathne, P. D., Dukovski, D. & Walz, T. The Affinity Grid: a pre-fabricated EM grid for monolayer purification. J. Mol. Biol. 382: 423-433 (2008).

2007

Buzhynskyy, N., Hite, R. K., Walz, T. & Scheuring S. The supramolecular architecture of junctional microdomains in native lens membranes. EMBO Rep. 8: 51-55 (2007).

Cheng, Y., Boll, W., Kirchhausen, T., Harrison, S. C. & Walz, T. Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly. J. Mol. Biol. 365: 892-899 (2007).

Park, H. H., Logette, E., Raunser, S., Cuenin, S., Walz, T., Tschopp, J. & Wu, H. Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex. Cell 128: 533-546 (2007).

Cheng, Y. & Walz T. Reconstructing the endocytotic machinery. Methods Cell Biol. 79: 463-487 (2007).

Ruch, C., Skiniotis, G., Steinmetz, M. O., Walz, T. & Ballmer-Hofer, K. Structure of a VEGF-VEGF receptor complex determined by electron microscopy. Nat. Struct. Mol. Biol. 14: 249-250 (2007).

Viadiu, H., Gonen, T. & Walz, T. Projection map of aquaporin-9 at 7 Å resolution. J. Mol. Biol. 367: 80-88 (2007).

Ohi, M. D., Ren, L., Wall, J. S., Gould, K. L. & Walz T. Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Proc. Natl. Acad. Sci. USA 104: 3195-3200 (2007).

Bankovich, A. J., Raunser, S., Juo, Z. S., Walz, T., Davis, M. M. & Garcia, K. C. Structural insight into pre-B cell receptor function. Science 316: 291-294 (2007).

Kelly, D. F., Lake, R. J., Walz, T.& Artavanis-Tsakonas, S. Conformational variability of the intracellular domain of Drosophila Notch and its interaction with Suppressor of Hairless. Proc. Natl. Acad. Sci. U. S. A. 104: 9591-9596 (2007).

Skiniotis, G., Moazed, D. & Walz, T. Acetylated histone tail peptides induce structural rearrangements in the RSC chromatin remodeling complex. J. Biol. Chem. 282: 20804-20808 (2007).

John, C. M., Hite, R. K., Weirich, C. S., Fitzgerald, D. J., Jawhari, H., Faty, M., Schläpfer, D., Kroschewski, R., Winkler, F. K., Walz, T., Barral, Y. & Steinmetz, M. O. The Caenorhabditis elegans septin complex is nonpolar. EMBO J. 26: 3296-3307 (2007).

Hite, R. K., Raunser, S. & Walz, T. Revival of electron crystallography. Curr. Opin. Struct. Biol. 17: 389-395 (2007).

Meijers, R., Puettmann-Holgado, R., Skiniotis, G., Liu, J. H., Walz, T., Wang, J. H. & Schmucker, D. Structural basis of Dscam isoform specificity. Nature 449: 487-491 (2007).

Norville, J. E., Kelly, D. F., Knight, T. F. Jr, Belcher, A. M. & Walz, T. 7Å projection map of the S-layer protein sbpA obtained with trehalose-embedded monolayer crystals. J. Struct. Biol. 160: 313-323 (2007).

Scheuring, S., Buzhynskyy, N., Jaroslawski, S., Gonçalves, R. P., Hite, R. K. & Walz, T. Structural models of the supramolecular organization of AQP0 and connexons in junctional microdomains. J. Struct. Biol. 160: 385-394 (2007).

Ohi, M. D., Feoktistova, A., Ren, L., Yip, C., Cheng, Y., Chen, J. S., Yoon, H. J., Wall, J. S., Huang, Z., Penczek, P. A., Gould, K. L. & Walz, T. Structural organization of the anaphase-promoting complex bound to the mitotic activator slp1. Mol. Cell 28: 871-885 (2007).

Onishi, M., Liou, G. G., Buchberger, J. R., Walz, T. & Moazed, D. Role of the conserved Sir3-BAH domain in nucleosome binding and silent chromatin assembly. Mol. Cell 28: 1015-1028 (2007).

2006

Hiroaki, Y., Tani, K., Kamegawa, A., Gyobu, N., Nishikawa, K., Suzuki, H., Walz, T., Sasaki, S., Mitsuoka, K., Kimura, K., Mizoguchi, A. & Fujiyoshi, Y. Implications of the aquaporin-4 structure on array formation and cell adhesion. J. Mol. Biol. 355: 628-639 (2006).

Cheng, Y., Wolf, E., Larvie, M., Zak, O., Aisen, P., Grigorieff, N., Harrison, S. C. & Walz, T. Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques. J. Mol. Biol. 355: 1048-1065 (2006).

Nakagawa, T., Cheng, Y., Sheng, M. & Walz, T. Three-dimensional structure of an AMPA receptor without associated stargazin/TARP proteins. Biol. Chem. 387: 179-187 (2006).

Mougous, J. D., Cuff, M. E., Raunser, S., Shen, A., Zhou, M., Gifford, C. A., Goodman, A. L., Joachimiak, G., Ordonez, C. L., Lory, S., Walz, T., Joachimiak, A. & Mekalanos, J. J. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312: 526-530 (2006).

Crampton, D. J., Ohi, M., Qimron, U., Walz, T. & Richardson, C. C. Oligomeric states of bacteriophage t7 gene 4 primase/helicase. J. Mol. Biol. 360: 667-677 (2006).

Nishida, N., Xie, C., Shimaoka, M., Cheng, Y., Walz, T. & Springer, T. A. Activation of leukocyte β2 integrins by conversion from bent to extended conformations. Immunity 25: 583-594 (2006).

Gonen, T. & Walz, T. The structure of aquaporins. Q. Rev. Biophys. 39: 361-396 (2006).

Kim, Y. G., Raunser, S., Munger, C., Wagner, J., Song, Y. L., Cygler, M., Walz, T., Oh, B. H. & Sacher, M. The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering. Cell 127: 817-830 (2006).

Fotin, A., Kirchhausen, T., Grigorieff, N., Harrison, S. C., Walz, T. & Cheng, Y. Structure determination of clathrin coats to subnanometer resolution by single particle cryo-electron microscopy. J. Struct. Biol. 156: 453-460 (2006).

Nishida, N., Walz, T. & Springer, T. A. Structural transitions of complement component C3 and its activation products. Proc. Natl. Acad. Sci. USA 103: 19737-19742 (2006).

2005

Ohi, M. D., Vander Kooi, C. W., Rosenberg, J. A., Ren, L., Hirsch, J. P., Chazin, W. J., Walz, T. & Gould, K. L. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol. Cell Biol. 25: 451-460 (2005).

Nakagawa, T., Cheng, Y., Ramm, E., Sheng, M. & Walz, T. Structure and different conformational states of native AMPA receptor complexes. Nature 433: 545-549 (2005).

Schmidt, M., Haas, W., Crosas, B., Santamaria, P. G., Gygi, S. P., Walz, T. & Finley, D. The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle. Nat. Struct. Mol. Biol. 12: 294-303 (2005).

Walz, T. How cholesterol-dependent cytolysins bite holes into membranes. Mol. Cell 18: 393-394 (2005).

Liou, G. G., Tanny, J. C., Kruger, R. G., Walz, T. & Moazed, D. Assembly of the SIR complex and its regulation by O-acetyl-ADP-ribose, a product of NAD-dependent histone deacetylation. Cell 121: 515-527 (2005).

Morikawa, M., Fryer, J. D., Sullivan, P. M., Christopher, E. A., Wahrle, S. E., DeMattos, R. B., O'Dell, M. A., Fagan, A. M., Lashuel, H. A., Walz, T., Asai, K. & Holtzman, D. M. Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-Î². Neurobiol. Dis. 19: 66-76 (2005).

Viadiu, H., Stemmann, O., Kirschner, M. W. & Walz, T. Domain structure of separase and its binding to securin as determined by EM. Nat. Struct. Mol. Biol. 12: 552-553 (2005).

Skiniotis, G., Boulanger, M. J., Garcia, K. C. & Walz, T. Signaling conformations of the tall cytokine receptor gp130 when in complex with IL-6 and IL-6 receptor. Nat. Struct. Mol. Biol. 12: 545-551 (2005).

Gonen, T., Cheng, Y., Sliz, P., Hiroaki, Y., Fujiyoshi, Y., Harrison, S. C. & Walz, T. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438: 633-638 (2005).

Cheng, Y., Zak, O., Aisen, P., Harrison, S. C. & Walz, T. Single particle reconstruction of the human apo-transferrin-transferrin receptor complex. J. Struct. Biol. 152: 204-210 (2005).

Smith, D. M., Kafri, G., Cheng, Y., Ng, D., Walz, T. & Goldberg, A. L. ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol. Cell 20: 687-698 (2005).

2004

Gonen, T., Sliz, P., Kistler, J., Cheng, Y. & Walz, T. Aquaporin-0 membrane junctions reveal the structure of a closed water pore. Nature 42: 193-197 (2004).

Luo, B. H., Strokovich, K., Walz, T., Springer, T. A. & Takagi, J. Allosteric β1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain. J. Biol. Chem. 279: 27466-27471 (2004).

Ray, S. S., Nowak, R. J., Strokovich, K., Brown, R. H. Jr., Walz, T. & Lansbury P.T. Jr. An intersubunit disulfide bond prevents in vitro aggregation of a superoxide dismutase-1 mutant linked to familial amytrophic lateral sclerosis. Biochemistry 43: 4899-4905 (2004).

Ohi, M., Li, Y., Cheng, Y. & Walz, T. Negative staining and image classification - powerful tools in modern electron microscopy. Biol. Proced. Online 6: 23-34 (2004).

Cheng, Y., Zak, O., Aisen, P., Harrison, S. C. & Walz, T. Structure of the human transferrin receptor-transferrin complex. Cell 116: 565-576 (2004).

Gonen, T., Cheng, Y., Kistler, J. & Walz, T. Aquaporin-0 membrane junctions form upon proteolytic cleavage. J. Mol. Biol. 342: 1337-1345 (2004).

Nakagawa, T., Futai, K., Lashuel, H. A., Lo, I., Okamoto, K., Walz, T., Hayashi, Y. & Sheng, M. Quaternary structure, protein dynamics, and synaptic function of SAP97 controlled by L27 domain interactions. Neuron 44: 453-467 (2004).

Fotin, A., Cheng, Y., Sliz, P., Grigorieff, N., Harrison, S. C., Kirchhausen, T. & Walz, T. Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature 432: 573-579 (2004).

Fotin, A., Cheng, Y., Grigorieff, N., Walz, T., Harrison, S. C. & Kirchhausen, T. Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating. Nature 432: 649-653 (2004).

2003

Kheterpal, I., Lashuel, H. A., Hartley, D. M., Walz, T., Lansbury, P. T. Jr. & Wetzel, R. Aβ protofibrils possess a stable core structure resistant to hydrogen exchange. Biochemistry 42: 14092-14098 (2003).

Lashuel, H. A., Hartley, D. M., Petre, B. M., Wall, J. S., Simon, M. N., Walz, T. & Lansbury, P. T. Jr. Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores. J. Mol. Biol. 332: 795-808 (2003).

Takagi, J., Strokovich, K., Springer, T. A. & Walz, T. Structure of integrin α5β1 in complex with fibronectin. EMBO J. 22: 4607-4615 (2003).

2002

Wang, L., Lashuel, H. A., Walz, T. & Colon, W. Murine apolipoprotein serum amyloid A in solution forms a hexamer containing a central channel. Proc. Natl. Acad. Sci. USA 99: 15947-15952 (2002).

Leggett, D. S., Hanna, J., Borodovsky, A., Crosas, B., Schmidt, M., Baker, R. T., Walz, T., Ploegh, H. & Finley, D. Multiple associated proteins regulate proteasome structure and function. Mol. Cell 10: 495-507 (2002).

Lashuel, H. A., Petre, B. M., Wall, J., Simon, M., Nowak, R. J., Walz, T. & Lansbury, P. T. Jr. &alpha-Synuclein, especially the Parkinson's disease-associated mutants, forms pore-like annular and tubular protofibrils. J. Mol. Biol. 332: 1089-1102 (2002).

Takagi, J., Petre, B. M., Walz, T. & Springer, T. A. Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell 110: 599-611 (2002).

Lashuel, H. A., Hartley, D., Petre, B. M., Walz, T. & Lansbury, P. T. Jr. Neurodegenerative disease: amyloid pores from pathogenic mutations. Nature 418: 291 (2002).

Cascio, P., Call, M., Petre, B. M., Walz, T. & Goldberg, A. L. Properties of the hybrid form of the 26S proteasome containing both 19S and PA28 complexes. EMBO J. 21: 2636-2645 (2002).

2001

Lederkremer, G. Z., Cheng, Y., Petre, B. M., Vogan, E., Springer, S., Schekman, R., Walz, T. & Kirchhausen, T. Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proc. Natl. Acad. Sci. USA 98: 10704-10709 (2001).

2000

Murata, K., Mitsuoka, K., Hirai, T., Walz, T., Agre, P., Heymann, J. B., Engel, A. & Fujiyoshi, Y. Structural determinants of water permeation through aquaporin-1. Nature 407: 599-605 (2000).

1999

Mitsuoka, K., Murata, K., Walz, T., Hirai, T., Agre, P., Heymann, J. B., Engel, A. & Fujiyoshi, Y. The structure of aquaporin-1 at 4.5-Å resolution reveals short α-helices in the center of the monomer. J. Struct. Biol. 128: 34-43 (1999).

1998

Walz, T., Jamieson, S. J., Bowers, C. M., Bullough, P. A. & Hunter, C. N. Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 Å, LH1 and RC-LH1 at 25 Å. J. Mol. Biol. 282: 833-845 (1998).

Hasler, L., Walz, T., Tittmann, P., Gross, H., Kistler, J. & Engel, A. Purified lens major intrinsic protein (MIP) forms highly ordered tetragonal two-dimensional arrays by reconstitution. J. Mol. Biol. 279: 855-864 (1998).

Walz, T. & Grigorieff, N. Electron crystallography of two-dimensional crystals of membrane proteins. J. Struct. Biol. 121: 142-161 (1998).

Hasler, L., Heymann, J. B., Engel, A., Kistler, J. & Walz, T. 2D crystallization of membrane proteins: rationales and examples. J. Struct. Biol. 121: 162-171 (1998).

1997

Cabiaux, V., Oberg, K. A., Pancoska, P., Walz, T., Agre, P. & Engel, A. Secondary structures comparison of aquaporin-1 and bacteriorhodopsin: a Fourier transform infrared spectroscopy study of two-dimensional membrane crystals. Biophys. J. 73: 406-417 (1997).

Walz, T., Hirai, T., Murata, K., Heymann, J. B., Mitsuoka, K., Fujiyoshi, Y., Smith, B. L., Agre, P. & Engel, A. The three-dimensional structure of aquaporin-1. Nature 387: 624-627 (1997).

Walz, T. & Ghosh, R. Two-dimensional crystallization of the light-harvesting I-reaction centre photounit from Rhodospirillum rubrum. J. Mol. Biol. 265: 107-111 (1997).

1996

Walz, T., Tittmann, P., Fuchs, K. H., Muller, D. J., Smith, B. L., Agre, P., Gross, H. & Engel, A. Surface topographies at subnanometer-resolution reveal asymmetry and sidedness of aquaporin-1. J. Mol. Biol. 264: 907-918 (1996).

Karrasch, S., Typke, D., Walz, T., Miller, M., Tsiotis, G. & Engel, A. Highly ordered two-dimensional crystals of photosystem I reaction center from Synechococcus sp.: functional and structural analyses. J. Mol. Biol. 262: 336-348 (1996).

Tsiotis, G., Walz, T., Spyridaki, A., Lustig, A., Engel, A. & Ghanotakis, D. Tubular crystals of a photosystem II core complex. J. Mol. Biol. 259: 241-248 (1996).

1995

Walz, T., Typke, D., Smith, B. L., Agre, P. & Engel, A. Projection map of aquaporin-1 determined by electron crystallography. Nat. Struct. Biol. 2: 730-732 (1995).

Walz, T., Haner, M., Wu, X. R., Henn, C., Engel, A., Sun, T. T. & Aebi, U. Towards the molecular architecture of the asymmetric unit membrane of the mammalian urinary bladder epithelium: a closed . J. Mol. Biol. 248: 887-900 (1995).

1994

Walz, T., Smith, B. L., Zeidel, M. L., Engel, A. & Agre P. Biologically active two-dimensional crystals of aquaporin CHIP. J. Biol. Chem. 269: 1583-1586 (1994).

Wu, X. R., Lin, J. H., Walz, T., Haner, M., Yu, J., Aebi, U. & Sun, T. T. Mammalian uroplakins. A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269: 13716-1324 (1994).

Walz, T., Smith, B. L., Agre, P. & Engel, A. The three-dimensional structure of human erythrocyte aquaporin CHIP. EMBO J. 13: 2985-2993 (1994).

1993

Dolder, M., Walz, T., Hefti, A. & Engel, A. Reconstitution of human erythrocyte band 3 into two-dimensional crystals. Soc. Gen. Physiol. 48: 63-76 (1993).

Dolder, M., Walz, T., Hefti, A. & Engel, A. Human erythrocyte band 3. Solubilization and reconstitution into two-dimensional crystals. J. Mol. Biol. 231: 119-132 (1993).